3R97
Crystal structure of malonyl-CoA:acyl carrier protein transacylase (FabD), Xoo0880, from Xanthomonas oryzae pv. oryzae KACC10331
Replaces: 3EENExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.425, 74.587, 98.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.260 - 2.300 |
| R-factor | 0.17102 |
| Rwork | 0.168 |
| R-free | 0.22528 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mla |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.791 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.270 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 18355 | |
| Completeness [%] | 92.7 | 88.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 287 | 0.1M CHES pH 9.0, 1.5M Ammonium sulphate, 0.2M Sodium chloride , VAPOR DIFFUSION, HANGING DROP, temperature 287K |
