3R8X
Crystal Structure of Methionyl-tRNA Formyltransferase from Yersinia pestis complexed with L-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 62.750, 62.750, 172.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.974 - 2.256 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fmt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.395 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_601)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 17092 | |
| <I/σ(I)> | 6.3 | 3.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M trimethylamine N-oxide, 0.1 M Tris, 20 % PEGMME2000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
