3R8J
Crystal structure of human SOUL protein (orthorhombic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 137.720, 114.660, 67.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.866 - 1.600 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.19670 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.065 | 0.380 |
| Number of reflections | 69655 | |
| <I/σ(I)> | 18.3 | 4.6 |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | KH2PO4 0.8M, NaH2PO4 0.8M, HEPES 0.08M, 1-butyl-3-methylimidazolium chloride 0.2M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
