3R7G

Crystal structure of Spire KIND domain in complex with the tail of FMN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2008-09-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	66.444, 66.444, 116.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.800 - 2.200
R-factor	0.19578
Rwork	0.194
R-free	0.22876
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	1.338
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.5.0093)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.200
Rmerge	0.081
Number of reflections	12200
Completeness [%]	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	PEG400, pH 6.0, VAPOR DIFFUSION, temperature 277K