3R6U
Crystal structure of choline binding protein OpuBC from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.900, 66.900, 64.100 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 18.690 - 1.610 |
| R-factor | 0.16263 |
| Rwork | 0.160 |
| R-free | 0.21016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sw2 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.092 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 30685 |
| Completeness [%] | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.7 | 285 | 20% PEG 3350, 0.2M potassium acetate, pH 8.7, VAPOR DIFFUSION, temperature 285K |
