3R5G
Crystal structure of the adenylyl cyclase CyaB from P. aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 51.360, 36.310, 94.760 |
| Unit cell angles | 90.00, 98.76, 90.00 |
Refinement procedure
| Resolution | 47.780 - 1.500 |
| R-factor | 0.18803 |
| Rwork | 0.186 |
| R-free | 0.23019 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wc1 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.367 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.364 |
| Number of reflections | 50946 | |
| <I/σ(I)> | 20.6 | 4.6 |
| Completeness [%] | 91.2 | 61.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1 M HEPES pH 7.5, 10 % (v/v) 2-Propanol, 20 % (w/v) PEG 4000, VAPOR DIFFUSION, temperature 298K |
