3R5E
TRANSALDOLASE from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.020, 78.860, 79.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.880 - 2.100 |
| R-factor | 0.19302 |
| Rwork | 0.189 |
| R-free | 0.26211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3clm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.161 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.880 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.067 | 0.165 |
| Number of reflections | 18584 | |
| <I/σ(I)> | 18.1 | 6 |
| Completeness [%] | 96.5 | 81.5 |
| Redundancy | 5.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 2.4 M ammonium sulfate, 0.1 M bis-Tris buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
