3R51
Structure analysis of a wound-inducible lectin ipomoelin from sweet potato
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.905, 118.070, 82.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.410 - 2.100 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r50 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.126 | 0.495 |
| Number of reflections | 17358 | |
| <I/σ(I)> | 21 | 6.1 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 11.5 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.0 M sodium chloride, 5% PEG 6,000 against a reservoir of 2.0 M sodium chloride, 10% PEG 6,000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
