3R51
Structure analysis of a wound-inducible lectin ipomoelin from sweet potato
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.905, 118.070, 82.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.410 - 2.100 |
R-factor | 0.184 |
Rwork | 0.184 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r50 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.126 | 0.495 |
Number of reflections | 17358 | |
<I/σ(I)> | 21 | 6.1 |
Completeness [%] | 99.5 | 100 |
Redundancy | 11.5 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.0 M sodium chloride, 5% PEG 6,000 against a reservoir of 2.0 M sodium chloride, 10% PEG 6,000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |