3R50
Structure analysis of a wound-inducible lectin ipomoelin from sweet potato
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 87.486, 139.468, 189.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.210 - 2.270 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ouw |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.082 | 0.457 |
| Number of reflections | 51599 | |
| <I/σ(I)> | 18.8 | 4.65 |
| Completeness [%] | 96.6 | 99.9 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.005 M ferric chloride, 0.05 M sodium citrate, 5% jeffamine M-600 against a reservoir of 0.01 M ferric chloride, 0.1 M sodium citrate, 10% jeffamine M-600, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
