3R4Y
Crystal structure of alpha-neoagarobiose hydrolase (ALPHA-NABH) from Saccharophagus degradans 2-40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9784 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.870, 76.810, 90.110 |
| Unit cell angles | 90.00, 101.86, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.000 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.627 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.086 | 0.460 |
| Number of reflections | 55856 | |
| <I/σ(I)> | 25.3 | 4.1 |
| Completeness [%] | 98.4 | 98.1 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 2.4M SODIUM MALONATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
