3R4H
Crystal structure of the 4-helix coiled coil CC-Tet-phi22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.840, 84.840, 58.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.420 - 2.700 |
| R-factor | 0.2042 |
| Rwork | 0.201 |
| R-free | 0.27003 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.321 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.160 | 0.871 |
| Number of reflections | 11164 | |
| <I/σ(I)> | 17 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 43.9 | 45.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1M Tris pH 7.5, 3M sodium formate, supplemented with 30% glycerol for cryo-protection, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
