3R4F

Prohead RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Detector	ADSC QUANTUM 270
Wavelength(s)	1.25465
Spacegroup name	I 4 2 2
Unit cell lengths	104.197, 104.197, 208.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.500
Rwork	0.239
R-free	0.27090
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.353
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXS
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Number of reflections	6608
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	100 mM MES, 10 mM magnesium diacetate, 2.5 M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	6	298	100 mM MES, 10 mM magnesium diacetate, 2.5 M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	6	298	100 mM MES, 10 mM magnesium diacetate, 2.5 M ammonium sulfate, pH 6.0, VAPOR DIFFUSION, temperature 298K