3R4A
Crystal structure of the 4-helix coiled coil CC-tet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 44.730, 50.900, 103.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.819 - 2.070 |
| R-factor | 0.2123 |
| Rwork | 0.209 |
| R-free | 0.26960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3R4H WITH IODOPHENYL GROUPS CHANGED TO TYROSINE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.773 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.030 | 2.180 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.102 | 0.624 |
| Number of reflections | 7490 | |
| <I/σ(I)> | 12.8 | 2.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2M lithium sulfate, 0.1M Tris pH 8.5, 1.26M ammonium sulfate, supplemented with 25% glycerol for cryo-protection , VAPOR DIFFUSION, SITTING DROP, temperature 291K |
