3R49
Human cyclophilin D complexed with quinolin-8-amine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-18 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.628, 56.628, 86.795 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.920 - 1.770 |
R-factor | 0.18823 |
Rwork | 0.186 |
R-free | 0.23568 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.978 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.4.0062) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 1.816 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 13514 | |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 30% PEG4000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |