3R48
Crystal structure of a hetero-hexamer coiled coil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.872, 50.850, 31.681 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 37.212 - 2.001 |
| R-factor | 0.2132 |
| Rwork | 0.210 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEMI-REFINED PDB ENTRY 3R46 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.854 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.098 | 0.398 |
| Number of reflections | 14338 | |
| <I/σ(I)> | 16 | 1.8 |
| Completeness [%] | 88.9 | 46.2 |
| Redundancy | 6.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2M sodium chloride, 0.1M sodium/potassium phosphate pH 6.5, 25% w/v PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
