3R3P
Homing Endonuclease I-Bth0305I Catalytic Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 52.292, 52.292, 133.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.198 - 2.200 |
R-factor | 0.2204 |
Rwork | 0.216 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.486 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 | 2.240 |
High resolution limit [Å] | 2.200 | 2.320 | 2.200 |
Number of reflections | 9728 | ||
Completeness [%] | 99.9 | 100 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 30% PEG 4000, 50 mM Tris, 100 mM LiSO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |