3R3O
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature and with high redundancy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | APEX II CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.132, 60.612, 37.632 |
| Unit cell angles | 90.00, 94.04, 90.00 |
Refinement procedure
| Resolution | 31.910 - 1.900 |
| R-factor | 0.1684 |
| Rwork | 0.163 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.338 |
| Data reduction software | APEX |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.033 | 0.266 |
| Number of reflections | 11067 | |
| <I/σ(I)> | 22.76 | 3.34 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.42 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
