3R3J

Kinetic and structural characterization of Plasmodium falciparum glutamate dehydrogenase 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97932
Spacegroup name	P 21 21 21
Unit cell lengths	138.780, 140.010, 180.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.990 - 3.100
R-factor	0.231
Rwork	0.231
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bma
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	CNS (1.2)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.200
High resolution limit [Å]	3.100	3.100
Rmerge		0.780
Number of reflections	64381
<I/σ(I)>	15.3	2.5
Completeness [%]	99.6	99.5
Redundancy	10.5	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M TRIS, 0.2M magnesium chloride hexahydrate, 30% PEG 4000, 0.01M spermine tetra HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K