3R2Q
Crystal Structure Analysis of yibF from E. Coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-04 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 111.340, 111.340, 111.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.000 - 1.050 |
R-free | 0.15280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.016 |
RMSD bond angle | 0.033 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.3.3) |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.090 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.069 | 0.465 |
Number of reflections | 107437 | |
<I/σ(I)> | 16.2 | |
Completeness [%] | 98.1 | 99.7 |
Redundancy | 7.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 298 | 5 microL protein in 50 mM HEPES pH 7.5, 1 mM DTT, 4 microL reservoir (0.1M Na acetate pH 4.6,1M ammonium phosphate monobasic), 1 microL 100mM glutathione pH 7.0, vapor diffusion, temperature 298K |