3R2G
Crystal structure of Inosine 5' monophosphate dehydrogenase from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | I 4 |
| Unit cell lengths | 115.131, 115.131, 63.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.700 - 1.941 |
| R-factor | 0.18069 |
| Rwork | 0.179 |
| R-free | 0.20671 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 29932 | |
| <I/σ(I)> | 13.2 | 3 |
| Completeness [%] | 97.6 | 83.5 |
| Redundancy | 17.9 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M NaCl, 0.1M Tris pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
