3R26
Perrhenate Binding to Molybdate Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.589, 81.589, 80.624 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.17316 |
| Rwork | 0.171 |
| R-free | 0.20535 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.030 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 32457 | |
| <I/σ(I)> | 24.3 | 6.6 |
| Completeness [%] | 84.4 | 84.4 |
| Redundancy | 11.1 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 27.5% PEG8000, 0.1M sodium acetate, 2.5M sodium perrhenate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
