3R1P

Odorant Binding Protein 7 from Anopheles gambiae with Four Disulfide Bridges, form P1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 1
Unit cell lengths	46.810, 57.992, 78.070
Unit cell angles	69.17, 85.91, 75.54

Refinement procedure

Resolution	38.830 - 1.850
R-factor	0.217
Rwork	0.216
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r1o
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.085	0.510
Number of reflections	59725
<I/σ(I)>	13.4	2.4
Completeness [%]	94.2	95
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.75	298	33 mM HEPES, pH 7.25-8.25, 0.4-1.0 M KCl, 6.6 mM sodium borate, 0.83 M sodium citrate tribasic, VAPOR DIFFUSION, SITTING DROP, temperature 298K