3R1P
Odorant Binding Protein 7 from Anopheles gambiae with Four Disulfide Bridges, form P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.810, 57.992, 78.070 |
| Unit cell angles | 69.17, 85.91, 75.54 |
Refinement procedure
| Resolution | 38.830 - 1.850 |
| R-factor | 0.217 |
| Rwork | 0.216 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r1o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.085 | 0.510 |
| Number of reflections | 59725 | |
| <I/σ(I)> | 13.4 | 2.4 |
| Completeness [%] | 94.2 | 95 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 33 mM HEPES, pH 7.25-8.25, 0.4-1.0 M KCl, 6.6 mM sodium borate, 0.83 M sodium citrate tribasic, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
