3R0Z
Crystal structure of apo D-serine deaminase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.020, 46.790, 100.040 |
| Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
| Resolution | 45.850 - 2.400 |
| R-factor | 0.21496 |
| Rwork | 0.212 |
| R-free | 0.27226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.437 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.109 | 0.492 |
| Number of reflections | 17806 | |
| <I/σ(I)> | 6 | 2.3 |
| Completeness [%] | 97.1 | 87.8 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 298 | 0.1M Trisodium citrate pH 6.1, 0.4M Ammonium sulphate, 0.8M Ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
