3R0S
UDP-N-acetylglucosamine acyltransferase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 98.959, 98.959, 157.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.400 - 2.300 |
| R-factor | 0.1978 |
| Rwork | 0.196 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j2z |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.300 | 50.000 | 2.260 |
| High resolution limit [Å] | 2.220 | 6.020 | 2.220 |
| Rmerge | 0.124 | 0.072 | 0.823 |
| Number of reflections | 14945 | ||
| <I/σ(I)> | 7.4 | 4.41 | |
| Completeness [%] | 99.3 | 99.1 | 98.8 |
| Redundancy | 24 | 22.7 | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 20% PEG MME 5000, 0.1 M Bis-tris buffer, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
