3R0G
3D Structure of Ferric Methanosarcina Acetivorans Protoglobin I149F mutant in Aquomet form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.844, 48.368, 80.782 |
| Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
| Resolution | 49.640 - 2.200 |
| R-factor | 0.24278 |
| Rwork | 0.239 |
| R-free | 0.31597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2veb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.133 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.810 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.112 | 0.283 |
| Number of reflections | 19625 | |
| <I/σ(I)> | 10.5 | 5.9 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 10% isopropanol, 20% PEG 4000, 0.1 M Na-Hepes, pH 7.5, VAPOR DIFFUSION, temperature 277K |
