3R0A
Possible transcriptional regulator from Methanosarcina mazei Go1 (gi 21227196)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.126, 67.059, 72.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.320 - 2.310 |
| R-factor | 0.27083 |
| Rwork | 0.266 |
| R-free | 0.36314 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.502 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 8825 |
| Completeness [%] | 78.6 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | Index screen, conditions #92 (H-8), 0.1M magnesium formate dihydrate, 15% w/v polyethylene glycol 3350, pH approximately 6.0 cryoprotected using Paratone-N, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
