3R05
Structure of neurexin 1 alpha (domains LNS1-LNS6), with splice insert SS3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2010-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.1272 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.030, 114.574, 160.129 |
| Unit cell angles | 89.60, 89.98, 87.96 |
Refinement procedure
| Resolution | 44.290 - 2.950 |
| R-factor | 0.2097 |
| Rwork | 0.208 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qcw |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.335 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
| Rmerge | 0.090 | 0.045 | 0.532 |
| Number of reflections | 89668 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 98.4 | 96.1 | 95.6 |
| Redundancy | 3.4 | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
