3R03
The crystal structure of NUDIX hydrolase from Rhodospirillum rubrum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.466, 107.466, 50.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.534 - 2.491 |
| R-factor | 0.2368 |
| Rwork | 0.231 |
| R-free | 0.28560 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.969 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 11862 | |
| <I/σ(I)> | 27.1 | 5.7 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 22 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M Sodium acetate trihydarate pH 4.6, 8% PEG 4000, 10 mM ATP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
