3QZY
Structure of Baculovirus Sulfhydryl Oxidase Ac92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-22 |
| Detector | ADSC Q315R |
| Wavelength(s) | 0.97692 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.730, 50.730, 65.170 |
| Unit cell angles | 83.95, 79.01, 65.58 |
Refinement procedure
| Resolution | 40.340 - 2.140 |
| Rwork | 0.231 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p0k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.044 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.340 | 2.260 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 31279 | |
| <I/σ(I)> | 8.5 | 3.1 |
| Completeness [%] | 97.6 | 95.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 4% PEG 2000 MME, 7% DMSO, 0.1M bicine, 0.05M arginine-HCl, 0.001M ZnCl2, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
