3QZX
3D Structure of ferric methanosarcina acetivorans protoglobin Y61A mutant with unknown ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.089, 49.239, 51.514 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 41.930 - 1.300 |
| R-factor | 0.13364 |
| Rwork | 0.132 |
| R-free | 0.16399 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.339 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.430 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.255 | |
| Number of reflections | 49084 | |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.4 monobasic ammonium phosphate, VAPOR DIFFUSION, temperature 277K |
