3QZ8
TT-4 ternary complex of Dpo4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.906, 102.694, 52.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.679 - 1.999 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jx4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.577 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 1.999 | 2.000 |
| Rmerge | 0.070 | 0.469 |
| Number of reflections | 35093 | |
| <I/σ(I)> | 26 | 1.3 |
| Completeness [%] | 94.7 | 58.2 |
| Redundancy | 6.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.04 | 293 | 12% PEG3350, 100 mM MES-Tris, 100 mM calcium acetate, 2.5% glycerol, pH 6.04, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
