3QZ7
T-3 ternary complex of Dpo4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-18 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.708, 102.461, 52.743 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.539 - 1.998 |
R-factor | 0.229 |
Rwork | 0.227 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jx4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.461 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 1.998 | 2.000 |
Rmerge | 0.050 | 0.346 |
Number of reflections | 33358 | |
<I/σ(I)> | 32.6 | 1.6 |
Completeness [%] | 89.9 | 31.9 |
Redundancy | 6.1 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 18% PEG3350, 100 mM HEPES, 100 mM calcium acetate, 2.5% glycerol, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |