3QY6
Crystal structures of YwqE from Bacillus subtilis and CpsB from Streptococcus pneumoniae, unique metal-dependent tyrosine phosphatases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.624, 59.641, 57.530 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.21836 |
| Rwork | 0.216 |
| R-free | 0.25573 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.553 |
| Number of reflections | 22595 | |
| <I/σ(I)> | 42.4 | 2.45 |
| Completeness [%] | 99.7 | 97.6 |
| Redundancy | 6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 273 | 100 mM Tris-HCl, 8% (w/v) PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
