3QY4
Crystallization and in situ data collection of Lysozyme using the Crystal Former
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-08-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.150, 79.150, 38.018 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.200 - 1.650 |
| R-factor | 0.17466 |
| Rwork | 0.173 |
| R-free | 0.21223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.665 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 4.460 | 1.650 |
| Rmerge | 0.059 | ||
| Number of reflections | 13594 | ||
| <I/σ(I)> | 2 | ||
| Completeness [%] | 90.0 | 62.3 | 94.3 |
| Redundancy | 2.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 298 | Crystals were grown through liquid-liquid diffusion using the Crystal Former from Microlytic, pH 4.6, microfluidic, temperature 298K |
