3QY2

Crystal structure of the P93A monomer mutant of S. cerevisiae Cks1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97740
Spacegroup name	P 43 21 2
Unit cell lengths	89.522, 89.522, 93.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.550 - 2.590
R-factor	0.21739
Rwork	0.211
R-free	0.27293
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.534
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.081	0.410
Number of reflections	12295
<I/σ(I)>	31.167	5.417
Completeness [%]	100.0	100
Redundancy		9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M sodium cacodylate, 0.2 M sodium citrate, 5% isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K