3QXQ
Structure of the bacterial cellulose synthase subunit Z in complex with cellopentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.678, 99.605, 93.073 |
| Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.200 |
| R-factor | 0.17076 |
| Rwork | 0.168 |
| R-free | 0.22742 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.870 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 81078 | |
| <I/σ(I)> | 2.65 | |
| Completeness [%] | 99.0 | 96.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 12-18% PEG3350, 50mM sodium citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
