3QWM

Crystal Structure of GEP100, the plextrin homology domain of IQ motif and SEC7 domain-containing protein 1 isoform a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97630
Spacegroup name	P 65 2 2
Unit cell lengths	56.730, 56.730, 234.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.070 - 2.390
R-factor	0.24369
Rwork	0.242
R-free	0.27242
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	1.288
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.6.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.070	2.520
High resolution limit [Å]	2.390	2.390
Rmerge	0.088	0.667
Number of reflections	9658
<I/σ(I)>	10.6	2
Completeness [%]	99.8	99.9
Redundancy	5.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.25	277	17.5%(w/v) PEG 3350, 0.20M Na nitrate, 10.0%(v/v) ethylene glycol, 0.1M Bis-Tris propane, pH 8.25, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	8.25	277	17.5%(w/v) PEG 3350, 0.20M Na nitrate, 10.0%(v/v) ethylene glycol, 0.1M Bis-Tris propane, pH 8.25, VAPOR DIFFUSION, SITTING DROP, temperature 277K