3QWL

Crystal structure of human TBC1 domain family member 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	34.574, 83.984, 87.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.213
Rwork	0.211
R-free	0.25850
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.322
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.066	0.040	0.855
Number of reflections	21049
<I/σ(I)>	11.4
Completeness [%]	99.7	97.6	100
Redundancy	7	6.2	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	20% PEG-1500, 0.2M magnesium chloride, 0.1M TRIS. 1:10 dispase-1 added in situ., pH 8.5, vapor diffusion, sitting drop, temperature 289K
2	VAPOR DIFFUSION, SITTING DROP	7.5	289	Selenomethionyl derivative protein. 30% PEG-1500, 0.2M sodium chloride, 0.1M HEPES. 1:10 dispase-1 added in situ., pH 7.5, vapor diffusion, sitting drop, temperature 289K