3QWE

Crystal structure of the N-terminal domain of the GEM interacting protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-21
Detector	RAYONIX MX-300
Wavelength(s)	0.92017
Spacegroup name	P 65 2 2
Unit cell lengths	57.530, 57.530, 500.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.2267
Rwork	0.227
R-free	0.23130
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	30.000	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.065	0.029	0.945
Number of reflections	20751	315	1445
<I/σ(I)>	24.19	51.6	2.2
Completeness [%]	99.9	96.6	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG-1500, 0.2M sodium chloride, 0.1M HEPES, 5% ethylene glycol, 3% glucose monohydrate. Crystals were de-hydrated by addition of 5% glycerol to reservoir solution, incubation over-night, pH 7.5, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG-1500, 0.2M sodium chloride, 0.1M HEPES, 5% ethylene glycol, 3% glucose monohydrate. Crystals were de-hydrated by addition of 5% glycerol to reservoir solution, incubation over-night, pH 7.5, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP