3QWD

Crystal structure of ClpP from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2008-06-18
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	172.048, 177.974, 100.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.990 - 2.100
R-factor	0.2086
Rwork	0.208
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.100
Number of reflections	179364
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	1.8 M ammonium sulfate and 100 mM sodium acetate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K