3QWB
Crystal structure of Saccharomyces cerevisiae Zeta-crystallin-like quinone oxidoreductase Zta1 complexed with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.961, 75.362, 182.833 |
| Unit cell angles | 90.00, 92.11, 90.00 |
Refinement procedure
| Resolution | 35.500 - 1.590 |
| R-factor | 0.18894 |
| Rwork | 0.188 |
| R-free | 0.21133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qor |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.106 | 0.157 |
| Number of reflections | 190318 | |
| <I/σ(I)> | 8.67 | 6.05 |
| Completeness [%] | 97.2 | 87.4 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 25% polyethylene glycol 3350, 0.1M Tris-HCl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.0K |
