3QVA
Structure of Klebsiella pneumoniae 5-hydroxyisourate hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.192, 101.302, 106.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.270 - 1.755 |
| R-factor | 0.1998 |
| Rwork | 0.199 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.171 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.272 | 50.000 | 1.820 |
| High resolution limit [Å] | 1.755 | 3.790 | 1.755 |
| Rmerge | 0.044 | 0.030 | 0.128 |
| Number of reflections | 45752 | ||
| <I/σ(I)> | 18.2 | ||
| Completeness [%] | 99.4 | 95 | 100 |
| Redundancy | 4.8 | 4.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M imidazole pH 7.5-8.5, 0.5 - 1.0 M ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
