3QUL
Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 altered peptide ligand (Y4S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9395 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.041, 122.697, 99.123 |
| Unit cell angles | 90.00, 103.33, 90.00 |
Refinement procedure
| Resolution | 51.780 - 2.000 |
| R-factor | 0.20898 |
| Rwork | 0.208 |
| R-free | 0.23441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1su7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.700 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.082 | 0.327 |
| Number of reflections | 150031 | |
| <I/σ(I)> | 13.2 | 3.9 |
| Completeness [%] | 89.9 | 56.3 |
| Redundancy | 3.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.8 M ammonium sulfate, 0.1 M Tris HCl pH 9.0., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
