3QUF
The structure of a family 1 extracellular solute-binding protein from Bifidobacterium longum subsp. infantis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935, 0.97921 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.532, 105.095, 145.375 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.270 - 1.700 |
| R-factor | 0.1523 |
| Rwork | 0.151 |
| R-free | 0.18040 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.081 | 0.060 | 0.582 |
| Number of reflections | 95082 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 98.1 | 98.8 | 95 |
| Redundancy | 4.6 | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.1M sodium acetate pH 4.6, 2.5M ammonium sulfate, 1/10 papain, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
