3QTP

Crystal Structure Analysis of Entamoeba histolytica Enolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.981
Spacegroup name	P 21 21 21
Unit cell lengths	64.420, 92.620, 160.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.310 - 1.900
R-factor	0.155
Rwork	0.153
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.900	20.000	1.900
Rmerge	0.094	0.021	0.400
Number of reflections	75593
<I/σ(I)>	12.75	34.32	3.38
Completeness [%]	98.8	85.7	92.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1M bis-Tris, 25% PEG 3350 (v/v), 0.2M (NH4)2SO4, pH 5,5, vapor diffusion, temperature 298K