3QT1
RNA polymerase II variant containing A Chimeric RPB9-C11 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-11-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.2664 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 222.380, 393.380, 281.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.600 - 4.300 |
| R-factor | 0.2363 |
| Rwork | 0.235 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.657 | 4.400 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Rmerge | 0.103 | |
| Number of reflections | 313148 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 98.4 | 99 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 200mM ammonium acetate, 300mM SODIUM ACETATE, 50mM Hepes pH 7.0, 5-6% PEG 6000, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
