3QSU
Structure of Staphylococcus aureus Hfq in complex with A7 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 3 |
| Unit cell lengths | 156.397, 156.397, 34.646 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.198 - 2.200 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.009 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.200 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 47823 | |
| Completeness [%] | 99.4 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12 % MPD, 0.1 M Na-cacodylate, 0.2 M Zn(0Ac)2, 0.1M KCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Na-cacodylate | ||
| 3 | 1 | 1 | Zn(0Ac)2 | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | Na-cacodylate | ||
| 7 | 1 | 2 | Zn(0Ac)2 | ||
| 8 | 1 | 2 | KCl |
