3QS3
Crystal structure of the biofilm forming subunit of the E. coli common pilus: donor strand complemented (DSC) EcpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 65 |
| Unit cell lengths | 101.790, 101.790, 387.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 88.170 - 2.100 |
| R-factor | 0.17034 |
| Rwork | 0.167 |
| R-free | 0.20019 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.476 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.170 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 115131 | |
| <I/σ(I)> | 8.1 | 1.8 |
| Completeness [%] | 97.4 | 98.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 20% PEG 3350, 200 mM Na Citrate , pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
