3QRX

Chlamydomonas reinhardtii centrin bound to melittin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	93
Detector technology	CCD
Collection date	2005-11-04
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	52.104, 114.426, 34.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.29364
Rwork	0.291
R-free	0.34074
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cdm
RMSD bond length	0.022
RMSD bond angle	1.753
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	10334
Completeness [%]	97.3	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2 L of the Crcen-MLT (1:1.5 molar ratio, 10mg/mL total) solution with 2 L of a precipitant solution containing 50 mM HEPES, pH 7.5, 0.2 M KCl, and 40% v/v pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 293K