3QRQ

Structure of Thermus Thermophilus Cse3 bound to an RNA representing a pre-cleavage complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	I 2 2 2
Unit cell lengths	61.500, 68.910, 158.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.884 - 3.194
R-factor	0.2129
Rwork	0.210
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.008
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (1.3.1)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.884	45.884	3.310
High resolution limit [Å]	3.194	6.890	3.194
Rmerge	0.168	0.072	0.412
Number of reflections	5725
<I/σ(I)>	5.5
Completeness [%]	97.6	99.7	89.4
Redundancy	7.8	10.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	vapour diffusion, hanging drop	7.5	298	15% PEG 3350, 100mM KOAc, 1mM spermidine, pH 7.5, vapour diffusion, hanging drop, temperature 298K